Optimal matching of hematopoitic cell transplant donors and recipients is based on multiple factors, but with a primary consideration given to the level of HLA genotype matching. Clinical complications post-transplant suggest that there is still much room for improvement in this matching process, and the additional HLA-allele level information provided by NGS typing should impact matching algorithms and improve overall patient outcomes long-term
Dr. Jennifer Schiller will be discussing the Next Generation Sequencing (NGS) technology and how it improves the donor selection within Histocompatability. Her discussion will lead into the analysis of NGS and how it can assist with virtual cross matching.
1) Describe the basics of next generation sequencing (NGS) technology
2) Explain some of the challenges of high resolution HLA typing
3) List at least 3 benefits of performing HLA typing by NGS for clinical testing